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Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications

By: Freshney, R. Ian.
Contributor(s): Capes-Davis, Amanda | Gregory, Carl | Przyborski, Stefan.
Material type: materialTypeLabelBookPublisher: Hoboken John Wiley & Sons 2016Edition: 7th ed.Description: xxxviii, 684p.ISBN: 9781118873656.Subject(s): Tissue Culture -- Laboratory Manuals | Cell Culture -- Laboratory ManualsDDC classification: 571.6381
Contents:
Table of Contents List of Figures, xix List of Color Plates, xxiii List of Tables, xxv List of Protocols, xxvii List of Minireviews, xxix Preface and Acknlowledgments, xxxi Abbreviations, xxxiii About the Companion Website xxxviii 1. Introduction, 1 2. Biology of Cultured Cells, 19 3. Laboratory Design and Layout, 37 4. Equipment and Materials, 51 5. Aseptic Technique, 73 6. Safety, Bioethics, and Validation, 89 7. Culture Vessels and Substrates, 111 8. Defined Media and Supplements, 125 9. Serum-Free Media, 149 10. Preparation and Sterilization, 173 10. Supplementary Material* 11. Primary Culture, 207 11. Supplementary Material* 12. Subculture and Cell Lines, 235 13. Authentication and Validation, 259 14. Microbial Contamination, 289 15. Cryopreservation and Banking, 307 16. Cloning and Selection, 327 16. Supplementary Material* 17. Cell Sorting, 347 18. Cell Line Characterization, 359 19. Differentiation, 383 20. Three ]Dimensional Culture 401 21. Scale ]up and Automation, 427 22. Senescence, Immortalization, and Transformation, 455 23. Quantitation, 483 23 Supplementary Material* 24. Cytotoxicity, 513 24. Supplementary Material* 25. Culture of Specific Cell Types, 531 25. Supplementary Material* 26. Stem Cells, 571 26. Supplementary Material* 27. Training Programs, 591 28. Problem Solving 627 29. In Conclusion, 651 Supplementary Material* Appendix III: Sources of Equipment and Material Appendix IV: Suppliers and Other Resource
Title is Recommended for the Course: New Arrivals Feb 2017
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Item type Current location Collection Call number Status Date due Barcode
General Book General Book Nava Nalanda Central Library
General Section
GEN 571.6381 FR-C (Browse shelf) Checked out 21/09/2017 130283
General Book General Book Nava Nalanda Central Library
General Section
GEN 571.6381 FR-C (Browse shelf) Checked out 21/09/2017 129302
General Book General Book Nava Nalanda Central Library
General Section
GEN 571.6381 FR-C (Browse shelf) Checked out 21/09/2017 129303

Table of Contents

List of Figures, xix

List of Color Plates, xxiii

List of Tables, xxv

List of Protocols, xxvii

List of Minireviews, xxix

Preface and Acknlowledgments, xxxi

Abbreviations, xxxiii

About the Companion Website xxxviii

1. Introduction, 1

2. Biology of Cultured Cells, 19

3. Laboratory Design and Layout, 37

4. Equipment and Materials, 51

5. Aseptic Technique, 73

6. Safety, Bioethics, and Validation, 89

7. Culture Vessels and Substrates, 111

8. Defined Media and Supplements, 125

9. Serum-Free Media, 149

10. Preparation and Sterilization, 173

10. Supplementary Material*

11. Primary Culture, 207

11. Supplementary Material*

12. Subculture and Cell Lines, 235

13. Authentication and Validation, 259

14. Microbial Contamination, 289

15. Cryopreservation and Banking, 307

16. Cloning and Selection, 327

16. Supplementary Material*

17. Cell Sorting, 347

18. Cell Line Characterization, 359

19. Differentiation, 383

20. Three ]Dimensional Culture 401

21. Scale ]up and Automation, 427

22. Senescence, Immortalization, and Transformation, 455

23. Quantitation, 483

23 Supplementary Material*

24. Cytotoxicity, 513

24. Supplementary Material*

25. Culture of Specific Cell Types, 531

25. Supplementary Material*

26. Stem Cells, 571

26. Supplementary Material*

27. Training Programs, 591

28. Problem Solving 627

29. In Conclusion, 651

Supplementary Material*

Appendix III: Sources of Equipment and Material

Appendix IV: Suppliers and Other Resource

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